Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata
نویسندگان
چکیده
We report the characterization of 16 polymorphic microsatellite markers in the golden apple snail, Pomacea canaliculata, a pest registered in the list of "100 of the world's worst invasive alien species". The fast isolation by AFLP (Amplified Fragment Length Polymorphism) of sequences containing repeats (FIASCO) method was used to isolate microsatellite loci, and polymorphism was explored with 29 individuals collected in an invasive region from China. These primers showed a number of alleles per locus ranging from three to 13. The ranges of observed and expected heterozygosity were 0.310-0.966 and 0.523-0.898, respectively. These microsatellite markers described here will be useful for population genetic studies of P. canaliculata.
منابع مشابه
Genetic Diversity of Introduced (pomacea Canaliculata) and Native (pila) Apple Snails in Thailand Revealed by Randomly Amplified Polymorphic Dna (rapd) Analysis
Genetic diversity of the introduced golden apple snail, Pomacea canaliculata (Lamarck, 1822) and four native apple snails; Pila ampullacea (Linneaus, 1758), P. angelica (Annandale, 1920), P. pesmei (Morelet, 1889) and P. polita (Deshayes, 1830) in Thailand were studied by RAPD analysis. Two hundred and two polymorphic fragments (180-1500 bp in length) were generated across overall investigated ...
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[This corrects the article DOI: 10.1186/s40064-016-3051-2.].
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